Chemical constituents from stems of Schisandra propinqua / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in the stems of Schisandra propinqua and evaluate their effects on proliferation of vascular smooth muscle cells (VSMCs) in vitro by MTT assay.</p><p><b>METHOD</b>The compounds were isolated and purified by various column chromatographic techniques and their structures were elucidated on the basis of spectral analysis (ESI-MS, 1H-NMR, 13C-NMR).</p><p><b>RESULT</b>Five compounds were isolated and identified as galgravin (1), veraguensin (2), octadecanoic acid 2, 3-dihydroxypropyl ester (3), hexadecanoic acid 2, 3-dihydroxypropy ester (4), tetracosanoic acid 2, 3-dihydroxypropyl ester (5).</p><p><b>CONCLUSION</b>All the compounds were isolated for the first time from the plant. 1, 2, 5 showed inhibiting effects on vascular smooth muscle cell (VSMCs) proliferation in vitro.</p>

Isolation and structure identification of chemical constituents from Polygala hongkongensis II / 中国中药杂志

<p><b>OBJECTIVE</b>To isolate and elucidate the constituents of herbs of Polygala hongkongensis.</p><p><b>METHOD</b>The constituents were isolated and purify by chromatographic on silica gel, Sephadax LH-20 and semi-preparative HPLC. The structures were determined by NMR and MS spectral analysis.</p><p><b>RESULT</b>Six compounds were identified as euxanthone (1), 1, 3, 6-trihydroxyxanthone (2), 1, 4, 7-trihydroxy-3-methoxy-xanthone (3), p-hydroxybenzoic acid (4), 3, 4-dihydroxybenzoic acid (5), and methy 1 2, 5-dihydroxybenzoate (6).</p><p><b>CONCLUSION</b>The known compounds 1-6 were isolated from this plant for the first time.</p>

The chemical constituents of Polygala hongkongensis Hemsl / 药学学报

Polygala hongkongensis Polycalaceae is mostly distributed in southern China, such as Guangdong, Jiangxi, Fujian and Sichuan provinces. And its herbs is used as a remedy of heat-clearing and detoxicating, removing food retention, promoting blood flow and expelling phlegm to arrest coughing in the folk medicine. Previous phytochemical investigations on Polygala plants have reported that the main chemical constituents are sapaonins, xanthones and oligosaccharide esters. To the best of our knowledge, there is no chemical report on the Polygala hongkongensis Hemsl. yet. In order to search and make use of natural resources from Polygala and to find the bioactive compounds and new compounds, we carried out studies on chemical constituents of this plant. The herbs of P. hongkongensis were extracted with 70% MeOH. The extract was combined and evaporated in vacuum to residue, which was suspended in water and successively partitioned with EtOAc and n-BuOH. Part of the n-BuOH extract was isolated and purified by various column chromatographs such as a macroporous resin, silica gel, Sephadex LH-20 column and semipreparative HPLC. The structures of isolated and purified compounds were determined by spectral analysis such as UV, IR, HRESI-MS, ESI-MS, 1H NMR, 13C NMR, HMQC, HMBC, H-H COSY, NOESY and physico-chemical property. Six compounds were identified as polyhongkonggaline (1), 3, 6'-di-O-sinapoyl-sucrose (2), tenuifoliside A (3), glomeratose D (4), cis-syringin (5), syringaresinol-4'-O-beta-D-monoglucoside (6). Compounds 1 is new compound, and 2-6 were isolated from this plant for the first time. Farther studies on the chemical constituents and pharmacological activities of P. hongkongensis will be carried out.

Pharmaphylogenetic study on isopyroideae (Ranunculaceae) / 中国中药杂志

<p><b>OBJECTIVE</b>To study the pharmaphylogenetic of medicinal plants of Isopyroideae (Ranunculaceae).</p><p><b>METHOD</b>Comprehensively analyze the correlation between phylogeny, chemical constituents and pharmaceutical aspects of Isopyroideae plants, based on chemical, pharmaceutical (both ethnopharmacologic and pharmacological) information, linking with different plant systems of Ranunculaceae.</p><p><b>RESULT</b>Plants from Aquilegia mainly contain flavonoids constituents while the major chemical constituents of Isopyrum are bisbenzylisoquinoline alkaloids. Chemical characteristics also support that this taxon should be separated from Thalictrodeae, and constituted an independent subfamily, namely, Isopyroideae.</p>

Preliminary pharmaphylogenetic study on Ranunculaceae / 中国中药杂志

<p><b>OBJECTIVE</b>To deal with the correlation between phylogeny, chemical constituents and pharmaceutical aspects of Ranunculaceae, namely a pharmaphylogenic study of this taxon.</p><p><b>METHOD</b>Based on chemical, pharmaceutical (both ethnopharmacologic and pharmacological) information, linking with different plant systems of Ranunculaceae.</p><p><b>RESULT</b>Chemical constituents of this taxon included several natural groups: benzylisoquinoline alkaloid, ranunculin, triterpenoid glycoside and diterpene alkaloid etc. Ranunculin and magoflorine were found to present simultaneously in some plants of this taxon.</p><p><b>CONCLUSION</b>Combining with therapeutic information, pharmaphylogenic research were in accordance with the phylogenetic system presented by Tamura that Ranunculaceae was proposed to be divided into six sub-families: Helleboroideae, Ranunculoideae, Cimicfugoideae, Isopyroideae, Thalictroideae and Coptidoideae. Results also supported the establishment of Cimicifugoideae.</p>

Cytotoxicity and mechanism of 23-O-acetylcimigenol-3-O-beta-D-xylopyranoside on HepG2 cells / 中国中药杂志

<p><b>OBJECTIVE</b>To elucidate the cytotoxicity and mechanism of 23-O-acetylcimigenol-3-O-beta-D-xylopyranoside isolated from C. dahurica on HepG2 cells and to find the leading compound for new drug development.</p><p><b>METHOD</b>MTT, AO/EB staining observation, flow cytometry and western blot methods were used to study the cytotoxicity, morphological changes, cell cycle distribution and protein expression profile of 23-O-acetylcimigenol-3-O-beta-D-xylopyranoside on HepG2 cells.</p><p><b>RESULT</b>23-O-acetylcimigenol-3-O-beta-D-xylopyranoside could inhibit the proliferation of HepG2 cells with IC50 at 16 micromol x L(-1), and could also induce apoptosis and G2-M cell cycle arrest. Further study demonstrated that the compound could cleavage PARP, regulate protein expression of bcl-2 family and decrease the expression of cdc 2 and cyclin B.</p><p><b>CONCLUSION</b>23-O-acetylcimigenol-3-O-beta-D-xylopyranoside exerts its cytotoxicity on HepG2 cells via apoptosis and G2-M arrest. In addition, caspases family activation, regulation of protein expression of bcl-2 family and down regulation of cdc 2 and cyclin B were involved in apoptosis and G2-M arrest induced by it.</p>

Chemical constituents in roots of Polygala fallax and their anti-oxidation activities in vitro / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in roots of P. fallax and their anti-oxidation activities in vitro.</p><p><b>METHOD</b>Column chromatographic techniques were employed for isolation and purification of chemical constituents of the plant. The structures were elucidated on the basis of the spectral evidence and the physical and chemical character. The isolated compounds were screened with four anti-oxidation models in vitro.</p><p><b>RESULT</b>Seven xanthones, 1,7-dihydroxy-2,3-methylenedioxyxanthone (1), 1-methoxy-2,3-methylenedioxyxanthone (2), 3-hydroxy-1,2-dimethoxyxanthone (3), 1,6,7-trihydroxy-2,3-dimethoxyxanthone (4), 7-hydroxy-1-methoxy-2,3-methylenedioxyxanthone (5), 1,3-dihydroxy-2-methoxyxanthone (6) and 1,3,7-trihydroxy-2-methoxyxanthone (7), were isolated from the roots of P. fallax. And compounds 1 - 7 showed different anti-oxidation activities in the different pharmacological models.</p><p><b>CONCLUSION</b>Compounds 2, 3, 5 and 7 were isolated from this plant for the first time. Xanthones from this plant showed anti-oxidation activities. The pharmacological activities of the pure compounds from this plant were also reported for the first time.</p>

Studies on TLC fingerprint of flavonoids in rhizome of Polygonatum odoratum / 中国中药杂志

<p><b>OBJECTIVE</b>To study the thin layer chromatographic (TLC) fingerprint of flavonoid constituents from Polygonatum odoratum, to set up the identification protocol of the herbal and provide scientific information for its quality control.</p><p><b>METHOD</b>The ethanol extracts were separated on silica gel G precoated plate with a mixture of toluene-ethylacetate-formic acid (5:4:1) as the mobile phase. The spots were visualized with ammonia vapor, then were examined under ultraviolet light (365 nm). The plate was scanned at wavelengths of lambdaR = 500 nm, lambdaS = 280 nm.</p><p><b>RESULT</b>A fingerprint of flavonoids of P. odoratum, with 10 specific fluorescent spots while examined under ultraviolet light, was set up.</p><p><b>CONCLUSION</b>The method can be used for quality control of P. odoratum.</p>

Content of inorganic elements of Salvia miltiorrhiza root in different area and physicochemical properties of its growing soil / 中国中药杂志

<p><b>OBJECTIVE</b>To provide basis of environmental factors of genuine crude drug, Salvia miltiorrhiza root.</p><p><b>METHOD</b>On-the-spot investigation and indoor chemical analysis were made to study the physicochemical properties of growing soil and content of inorganic elements of S. miltiorrhiza, and obtained data were analysed with SPSS 10.0 software.</p><p><b>RESULT</b>S. miltiorrhiza root of high harvest area accumulated Cu and Zn. In soil principal component analysis, no principal component was obvious. So the drug has good adaptability in ecological environment of soil.</p><p><b>CONCLUSION</b>Ecological environment of soil isn't leading factor in forming genuine crude S. miltiorrhiza.</p>

Study on the physicochemical properties of cultivated soil of genuine crude and no-enuine crude Chinese Angelica / 中国中药杂志

<p><b>OBJECTIVE</b>To provide basis for environmental factors of genuine crude Chinese angelica.</p><p><b>METHOD</b>On-the-spot investigation and indoor chemical analysis were made to study the physicochemical properties of cultivated soil of Chinese angelica.</p><p><b>RESULT AND CONCLUSION</b>The physical properties and the organic matte and mineral nutrition of cultivated soil of Chinese angelica are best in Min County, Gansu Province. The ecological environment is the leading factor in forming genuine crude Chinese angelica.</p>